colorimetric ldh activity assay kit  (Elabscience Biotechnology)

Journal: Biotechnology and bioengineering

Article Title: Assessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform.

doi: 10.1002/bit.29033

Figure Lengend Snippet: FIGURE 3 | Schematic representation of the experimental design for two‐dimensional (2D) and three‐dimensional (3D) cell culture studies. LDH, lactate dehydrogenase; MTT, 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide.

Article Snippet: The cell damage was evaluated by the LDH quantity released into the culture medium by the colorimetric lactate dehydrogenase (LDH) activity test (Elabscience, USA).

Techniques: Cell Culture

Journal: Biotechnology and bioengineering

Article Title: Assessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform.

doi: 10.1002/bit.29033

Figure Lengend Snippet: FIGURE 9 | Evaluation of cellular responses in 3D spheroids treated with different concentrations of Doxorubicin (Dox 10, Dox 5) and Aloe vera (AV 100, AV 50) over a 10‐day culture period. (A) Cell viability inhibition measured via Alamar Blue assay, showing significant reductions in viability across treatment groups compared to control, with Doxorubicin causing a more rapid and pronounced inhibition. (B) LDH release into the culture medium as an indicator of membrane damage, with Doxorubicin causing a significant increase in LDH levels, indicative of necrosis, while Aloe vera induced a more gradual release, consistent with apoptotic cell death. (C) Remaining glucose levels in the culture medium, highlighting reduced glucose consumption in the Doxorubicin‐treated spheroids due to cell death, while Aloe vera‐treated spheroids showed a delayed but progressive reduction in glucose consumption. (D) Albumin secretion as a measure of liver function, with Doxorubicin causing a rapid decline in albumin production, and Aloe vera exerting a more gradual inhibitory effect on metabolic activity. Data are presented as mean ± standard deviation (n = 3). Statistical significance shown was evaluated using one‐way ANOVA followed by Tukey's post hoc test. Significant differences compared to the control group at each time point are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, **p < 0.0001, and ns = not significant. ANOVA, analysis of variance; LDH, lactate dehydrogenase.

Article Snippet: The cell damage was evaluated by the LDH quantity released into the culture medium by the colorimetric lactate dehydrogenase (LDH) activity test (Elabscience, USA).

Techniques: Inhibition, Alamar Blue Assay, Control, Membrane, Activity Assay, Standard Deviation